I Now Have 3500 Miles On My 06 R1. I Want To Go -1 Front And +1 On The Rear. My Question Is Can I Use A Chain With 3500 Mi. On New Sprockets Without Any Problems Or Do I Need A New Chain Also?

If you've taken care of it, the chain shouldn't have any wear and should have a lot of life left. If not and you replace the chain, might as well go with a 520 conversion.

most people will tell you to get new chain. old chain will wear new sprockets faster. but if you have taken good care of your chain.. then its worth a shot.

measure the chain and then compare with the service manual and see how stretched it got. or just put it next to a new one and see. I had a 900 rr rear sprocket that had 15k miles on it and the sprocket was good as new and it was even measured and lost very little.